Using Hydrogen Peroxide for Reducing Bacterial Contamination in Date Palm Tissue Culture

Abstract

The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to
bacterial contamination which usually occurs in the micropropagation of date palm. Different
concentrations of hydrogen peroxide at different time intervals were examined and compared to ethyl
alcohol and distilled water with both of callus and shoot explants of date palm cv. Sewi. The most
powerful disinfection with 100% survival occurred by using 10 ppm hydrogen peroxide for 1 min.