Effect of the rhizobacterium Bacillus subtilis on the development of the root-knot nematode Meloidogyne arenaria at different temperatures

Abstract

The root-knot nematode Meloidogyne arenaria was cultured in a controlled environment to determine the
influence of temperature on the efficiency of the rhizobacterium Bacillus subtilis. Seedlings of the second
stage of the Mycobacterium arenaria were introduced to potato plants (Solanum tuberosum L.). Bacterial
suspensions of two local strains of B. subtilis (A1 and B1) were applied to the plant rizosphere on the 7th,
14th, and 21st days following worm inoculation. The potato plants were cultivated in a controlled
environment with temperatures ranging from 16±1 to 32±1ºC. After treating potato roots with B. subtilis
(strains A1 and B1), the rate of M. arenaria development was shown to be reduced compared to untreated
plant roots. Under these conditions, the bacteria inhibited the growth of Mycoplasma arenaria to the greatest
extent